The effect of anti-VEGF antiserum on the progression of bladder cancer in mouse model / 中华泌尿外科杂志

ABSTRACT

Objective To investigate the effect of antiserum against VEGF on angiogenesis and growth of bladder cancer in a mouse model in order to explore a novel strategy for the treatment of bladder cancer. Methods Antiserum against VEGF was prepared by using recombinant human VEGF and was identified for its specific affinity with VEGF by ELISA.The bladder carcinoma cell lines BST739 were implanted in T739 mice which were randomly divided into 2 groupsthe control group receiving normal mice serum intraperitoneally,and the experimental group receiving antiserum against VEGF intraperitoneally.All the animals were sacrificed at last.Then all the bladder carcinomas were weighted and the microvessel density (MVD) in them was studied by immunohistochemical staining. Results The antiserum against VEGF was prepared and demonstrated with high specificity. The MVD in the antiserum group was lower than that in the control group. But the average weight of tumors was not significantly less in the antiserum group. Conclusions The study shows that to inhibit the activity of VEGF may reduce the angiogenesis in bladder cancer and supports the antiangiogenic therapy for bladder cancer.Inhibition of VEGF activity might become an important way of adjuvant therapy for bladder cancer.