Differentiation of rat bone marrow stromal cells into Schwann cells-like in vitro / 中华显微外科杂志
Chinese Journal of Microsurgery
;
(6)1998.
Article
in Chinese
| WPRIM
| ID: wpr-537695
ABSTRACT
Objective To study the protocol that rat bone marrow stromal cells (BMSCs) can be induced to differentiate into Schwann cell-like in vitro. Methods BMSCs were treated with beta-mercaptoethanol followed by retinoic acid and cultured in the presence of forskolin,basic-FGF,PDGF and heregulin. The positive percentages of GFAP,S-100 protein expression were measured by immunocytochemistry SABC staining. Results After the induction,BMSCs displayed morphologies of Schwann cell,such as ellipsoidal cell bodies and fences-like array,with two or three sligh cell processes. The positive percentages of GFAP protein expression was from 42.5% to 68.7%,S-100 was from 39.6% to 60.2%. Conclusions Rat BMSCs could be induced to Schwann cell-like by beta-mercaptoethanol,retinoic acid,forskolin,basic-FGF,PDGF and heregulin
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Microsurgery
Year:
1998
Type:
Article
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