Injectable tissue-engineered bone using calcium alginate as carriers / 实用口腔医学杂志
Journal of Practical Stomatology
;
(6)1996.
Article
in Chinese
| WPRIM
| ID: wpr-537816
ABSTRACT
objective:
To develop injectable tissue engineered bone through injection of osteoblasts/alginate composite in rabbits.Methods:
Bone marrow cells isolated from iliac bone of New Zealand rabbits were cultured and induced to differentiate into osteoblasts.The osteoblasts were mixed with 25 g/L sodium alginate solution to generate osteoblasts/alginate composite with final cellular density of 5?10 6/ml. 0.17 g of sterilized CaSO 4 powder was then added to 2 ml osteoblasts/alginate. The mixture was injected into the dorsal subcutaneous tissue at left side of 6 New Zealand rabbits. The alginate composite without osteoblasts was injected into the right side as the control. 4 and 8 weeks after implantation, the bone formation was evaluated by means of gross, X ray and histological observation.Results:
4 weeks after implantation, cartilage formation was observed and 8 weeks after implantation,new mature bone was observed in the osteoblasts/alginate composites. No new bone formation was observed in all of the control specimens.Conclusion:
Calcium alginate can be used as a carrier in injectable bone tissue engineering, and new bone can be created through injection of osteoblasts/alginate composites in immune animals.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Practical Stomatology
Year:
1996
Type:
Article
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