Proliferation of cultured skin fibroblast after low intensity pulsed ultrasound exposure / 重庆医科大学学报

ABSTRACT

Objective:To observe the effects of different low intensity pulsed ultrasound(LIPUS) on proliferation of fibroblast and to screen out the optimum parameters of LIPUS.Methods:LIPUS of different frequency(1?1.2?1.7MHz)and different intensity was used to stimulate the cultured human fibroblast cells for 5 min,10 min,20 min and 30min,respectively,and examination of cell proliferation by MTT after 24h.Results:①Optical absorption value of cell activity in MTT assay increased significantly in the cell culture with LIPUS of different frequency(1?1.2?1.7MHz) and four intensity of each frequency treatment for 5,10 minutes,as compared with the control group(P