FⅤ Leiden mutation,the anticardiolipin antibodies and ischemic cerebrovascular disease in Chinese patients / 中华神经科杂志

ABSTRACT

Objective To study the prevalence of FⅤ Leiden mutation and its relationship to anticardiolipin antibodies (aCL) and ischemic cerebrovascular disease (ICVD). Methods The factor Ⅴ Leiden mutation was analyzed in 75 healthy and 118 ICVD patients using PCR-RFLP analysis.The anticardiolipin antibodies level was also measured by enzyme-linked immunosorbent assay along with international standards in 86 patients with ICVD.The resistance to activated protein C(APC-R )was measured in 27 patients. Results The mutation, in heterozygous form, was found in 6 cases out of 118 ICVD patients, with a prevalence of 5.1%.None of the 75 healthy were found to carry the FⅤ Leiden mutation.The patients who carried the FⅤ Leiden mutation were young, whose average age was 50 years old.In 27 patients whose APC-R was measured , 8 patients were positive.In 86 patients whose aCL level was also measured simultaneously, an elevated aCL level was found in 26 cases, of whom 3 cases were found carrying the FⅤ Leiden mutation as well, making the group of patient possess a prevalence of 11.5 %, showing no strongly statistical significance in contrast to the patients without elevated aCL 5.0%.However, within the total of 6 cases with FⅤ Leiden mutation found in this group, 3 cases were patients with elevated aCL.Conclusions It was suggested that although FⅤ Leiden mutation was accounted for a small proportion of patients with ICVD, it might play a crucial role in thrombosis. The prevalence of young patients who carried FⅤ Leiden mutation should be higher.The final combined analysis revealed FⅤ Leiden mutation which is of highly significant relation to aCL in this group of Chinese patients with ICVD.It's detection should be of greatly clinical significance in finding cause, predicting the progress, prevention and treatment.