The role of transcription factor NF-kB in the INS-1 cell impairment induced by IL-1? and other cytokines / 中华内分泌代谢杂志

ABSTRACT

Objective To investigate the role of NF-kB in cytokines (IL-1?, TNF-? and IFN-r) -induced impairment of INS-1 cell, a pancreatic islet ?-cell line. Methods Retrovirus expressing IkB??N with mutant IkB?(inhibitor of NF-kB ) was constructed. Immunoblot analysis, fluorescence analysis using a kB-1uc reporter gene and thiazolyl blue viability assay were applied in this study. Results IL-1?-induced IkB? degradation and NF-kB activation were found in INS-1 cells but not in INS-1/IkB??N cells infected with IkB??N retrovirus. The viability of INS-1 cells incubated with the combination of IL-1? and IFN-r or TNF-? and IFN-r was decreased but INS-1/IkB??N cells could resist these cytokines-induced decrease of cell viability. Conclusion IL-1?, TNF-?can decrease the viability of? cells, while IFN-r may have some enhancing role. Inhibition of NF-kB activation can protect ? cells from the cytotoxicity of the cytokines.