Experimental study of the early period of fracture healing in a rat osteoporotic model prepared by ovariecto-my / 中华骨科杂志

ABSTRACT

Objective The research was to observe the changes of histomorphology,bone mineral density of callus and the expression and distribution of transforming growth factor beta(TGF-? 1 ),basic fi-broblast growth factor(bFGF)and bone morphogenetic proteins-2(BMP-2)in osteoporotic fracture so as to evaluate the influence of osteoporosis on fracture healing process.Methods An osteoporotic animal model was established by ovariectomy in sixty SD rats,in which the femur was osteotomized by a wire saw as frac-ture healing model,internal fixed with a Kirschner pin three months after ovariectomy.The rats were scari-fied in3days,1,2,4and6weeks after operation,the callus formation was monitored over a6weeks period by histological and immunohistochemistry assessment.Results The status of osteoporosis in ovariectomy group was confirmed by total body bone mineral density measurement 3months after ovariectomy.The aver-age body weight of ovariectomy animals was337.8g compared to286.2g in the control group(P