Purification of human TRAIL extracellular region and its activity / 中国免疫学杂志
Chinese Journal of Immunology
;
(12)2000.
Article
in Chinese
| WPRIM
| ID: wpr-538336
ABSTRACT
Objective:
To express hTRAIL_ 41~281 protein using E.coli and refold it into a functional form.Methods:
The expression of protein was induced by IPTG,protein was purified by Ni-NTA chromatography column,and refolded by dialysis ,protein purified was determined by SDS-PAGE and Western blot.Antitumor activity of hTRAIL_ 41~281 protein was measured by DNA fragmentation electropherogram.Results:
Results of SDS-PAGE and Western blot proved that the 30.5 kD protein purified were hTRAIL_ 41~281 protein,the purity of protein was more than 90%.After refolding,DNA fragmentation electropherogram showed that hTRAIL_ 41~281 protein had good antitumor activity.Conclusion:
hTRAIL_ 41~281 protein with antitumor activity was successfully expressed with E.coli and purified by Ni-NTA chromatography column,refolded by dialysis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
2000
Type:
Article
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