Doppler tissue imaging of porcine chronic ischemic myocardium before and after gene transfer / 中华超声影像学杂志

ABSTRACT

Objective To investigate the value of Doppl er tissue imaging (DTI) in detecting chronic ischemic myocardium before and after gene transfer. Methods An Ameroid constrictor was placed around the left circumflex artery of each open-chest pig. After 4 weeks, groupⅠandⅡwere intramyocardially administrated recombinant adenovirus coding for the vascular endothelial growth factors(Ad.VEGF-B) or phosphate buffer(PB). After 4 weeks, their hearts were removed for pathological investigation. Color M-mode, two-dimensional and spectrum DTI of lateral-posterior wall before operation, 2 weeks and 4 weeks after Ameroid placement, 2 weeks and 4 weeks after administration were studied. Results Color of normal myocardium was bright, filling and distinct stratified, and of ischemic myocardium faint, local lost and cloudy stratified. After gene transfer, color filling became better and stratification distinct. Normal V S of apical four chamber view (AP-4CV) was greater than that of left ventricular papillary muscle level short axis view (LVPM-SAM)(P