Effect of T_3 on expression of thyroid hormone rece ptor mRNA during differentiation of human neural stemcell / 中华内分泌代谢杂志

ABSTRACT

Objective To ex pl ore the action of T 3 on neural stem cell (NSC) differentiation and the change of thyroid hormone receptor (TR) mRNA expression during NSC differentiation. Methods Human embryonic brain-derived NSC was suc cessfully expanded into neurospheres with mitogens and identified by immunocytoc hemistry method. T 3 was used to induce the differentiation of NSC. During the NSC differentiation, mRNA expression levels of different TRs were detected by se miquantitative RT-PCR. At the same time the cell types of the differentiated ce lls were identified by immunocytochemistry method. Results After being induced by T 3, NSC could differentiate into neuron, ol igodendrocyte, astrocyte, but myelin basic protein positive cells accounted for 80%. The time sequence expression of different TRs mRNAs existed during NSC diff erentiation. Conclusion T 3 can induce NSC to develop to glial cell and is an important regulator of the oligodendrocyte diffe nrentiation. T 3 exerts distinct effects through time sequence expressions of d ifferent TRs mRNAs.