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The experimental study of transferring the hTERT gene / 西安交通大学学报(医学版)
Journal of Xi'an Jiaotong University(Medical Sciences) ; (6)2003.
Article in Chinese | WPRIM | ID: wpr-538932
ABSTRACT
Objective To establish the human telomerase reverse transcriptase (hTERT) gene viral transferring system. Methods By means of liposome mediation, a retroviral vector pLNC-hTERT carrying a selectable marker neomycin resistance genes (Neo r) and a target gene (hTERT) was transferred into the ectropic package cells ?-2, and by using the supernatant of ?-2 cells to infect the amphotropic producer clone PA317, established PA317/hTERT package cell line. In order to get higher-titer virus, the supernatants of ?-2 infected PA317 repeatedly. Human endothelial cells were used to detect the effect of gene transfer. Results We established a PA317/hTERT package cell line which produced high-titer virus. The viral titer produced by PA317 cells was raised 20 times by repeated infection method, and with higher-titer virus the exogenous genes were successfully transferred into endothelial cell. Conclusion This method can be used to establish higher-titer viral transferring system.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Xi'an Jiaotong University(Medical Sciences) Year: 2003 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Xi'an Jiaotong University(Medical Sciences) Year: 2003 Type: Article