Allogeneic transplantation of myoblasts seeded on typeⅠcollagen sponges in rats / 实用口腔医学杂志
Journal of Practical Stomatology
;
(6)2001.
Article
in Chinese
| WPRIM
| ID: wpr-539046
ABSTRACT
s Objective:
To study the feasibility of allogeneic tra ns plantation of myoblasts of SD rats for the reconstruction of facial muscle de fects.Methods:
Myoblasts obtained from the forelimbs and hindlimbs of neonatal SD rats were purified and cultured. 5?10 4 myoblasts w ere seeded onto each of type Ⅰcollagen sponges in the size of 1.5 mm?0.8 mm? 0.5 cm. The myoblasts/sponge constructs were transplanted into the facial muscl e defects of syngeneic mutured rats and observed with microscopy,immunohistoche mistry and electromyography (EMG).Result:
Myoblasts attac hed and kept alive on type I collagen for at lest 3~4 days in culture. 4 and 8 w eeks after transplantation,muscle like tissue with blood vessles was observed i n the implants. Expression of actin and myosin in the implants was similar to th at in the control muscle.8 weeks after implantation,the spike wave (mV) on the g rafted side and control side was 0.7?0.3 and 2.7?0.6 ( P
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Practical Stomatology
Year:
2001
Type:
Article
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