Effect of 8-Br-cAMP and IPP on the expression of tau protein in various neural cell lines / 中华老年医学杂志
Chinese Journal of Geriatrics
;
(12)1995.
Article
in Chinese
| WPRIM
| ID: wpr-539088
ABSTRACT
ObjectiveTo study the effect of 8-Br-cAMP and isopentenyl pyrophosphate (IPP) on the ex pression tau protein in various neural cell lines at the level of signal transdu ction. MethodsNG108-15, PC12 and SH-SY5Y cells were treated with 8-Br-cAMP and IPP in the 37?C incubator for 30 min, then the tau-enriched samples were analyzed by West ern blot. ResultsSH-SY5Y cells only expressed the isoform of tau protein with the lowest molecul ar weight; PC12 cells mainly expressed high molecular weight tau, and several ot her isoforms of tau; NG108-15 expressed a little of isoform of tau. In the expe rimental conditions, the expression of tau was altered with 8-Br-cAMP and IPP in NG108-15, SH-SY5Y and PC12 cells. Conclusions8-Br-cAMP and IPP does not alter the expression of tau in NG108-15, SH-SY5Y and PC12 cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Geriatrics
Year:
1995
Type:
Article
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