A lungs-specific liposomal delivery system for gene therapy / 中国免疫学杂志

ABSTRACT

Objective:To construct and evaluate a Salmeterol lungs-specific liposomal delivery system.Methods:①Liposome were prepared by the reverse-phase evaporation method and instilled into the tracheas of SD strain rats.The influence of Salmeterol on the uptakes of liposome was evaluated.②pEGFPC1(25 ?g/rodent) encapsulated in lungs-specific liposome were administered intravenously into Guinea pigs,GFP expression were observed by means of fluorescent microscopy 24h and 48h after administration.Results:①2,4,8,12,16 hours after instillation,lungs-specific liposome uptake were significantly higher than nonspecific liposome uptake(P