The application of STR loci genescan in paternity testing / 西安交通大学学报(医学版)

ABSTRACT

Objective To search for an easy standardization p aternity testing method. Methods Based on nine completed da ta and stable tetranucleotide STR loci, 45 paternity cases were typed by using f luorescence labeling primer, multiplex PCR, higher sensitivity 377 DNA sequencer , and then the computer automatically collected the data and made analysis, STR loci alleles and genotypes were determined. Results Th ere was no relationship between alleged fathers and children in 21 cases. But in 24 cases the relationship could not be excluded. Conclusion The STR loci genescan method is convenient, time-saving, fast and has hig her sensitivity. The results are accurate and reliable.