Determination of Chromium in Human Hair by Spectrophotometry / 环境与健康杂志

ABSTRACT

Objective To study the determination of chromium in human hair by spectrophotometry using starch as color agent. Methods The total chromium(Cr)in human hair was oxidized by (NH4?雪2S2O3 into Cr6+,which could oxidize KI to form I-3 in acid medium. I-3 reacted with starch to form a blue colored complex,which revealed the maximum absorption peak at 590 nm. Results The contents of Cr in human hair were 8.46-29.56 ?g/g.The lowest detection limit and linear range were 0.02 and 0.02-1.0 ?g/ml respectively.The coefficient of variation and standard material-added recovery rates were 3.3% and 94.8%-104.5% respectively. Conclusion This methods was rapid,accurate and convenient for the determination of chromium in human hair.