Effect of different substrates coated on spinal motor neurons from embryonic rat cultured in vitro / 中国免疫学杂志

ABSTRACT

Objective:To study the effect of different substrates coated on the cell survival and neurite outgrowth of spinal motor neurons (SMN) from embryonic rat cultured in vitro.Methods:The ventral spinal tissue was isolated from embryonic rats and digested into dissociated cell suspention for culture,then the cells were identified as SMN by immunohistochemistry stain.Poly-L-lysine(PLL) was dissolved into distilled water,phosphate-buffered saline solution,boric acid at 0.1 and 0.01 mol/L concentration respectively.The different substrates include PLL,collagen Ⅰ,laminin and PLL combined with laminin.Distilled water was used as control.The neuron survival numbers and the mean length of the neurites were measured and compared.Results:The cells on the PLL dissolved into boric acid at 0.01 mol/L concentration survived well.The SMNs grown on the PLL combined with laminin were in dispersed disitribution with high survival rate.Conclusion:PLL combined with laminin is the best for the study of the motor neuron including both soma and neurite.