The effects of presenilin-1 mutation on the proliferation and apoptosis of all-trans retinoic acid(RA) -induced PC12 cells / 中华老年医学杂志
Chinese Journal of Geriatrics
; (12)2003.
Article
in Zh
| WPRIM
| ID: wpr-539968
Responsible library:
WPRO
ABSTRACT
Objective To investigate the effects of mutated PS-1 on the proliferation and apoptosis of RA-induced PC12 cells . Methods The MTT assay, flow cytometry, TUNEL method were used in the research. Results An inhibition of proliferation was demonstrated in the RA-induuced PC12 cells expressing PS-1 mutant L286V. The proliferation of the cells was blocked in the G1→S phase. Whether with or without fetal bovine serum(FBS), PS-1 mutant L286V was able to accelerate the apoptosis of RA-induced PC12 cells. This action was enhanced markedly under the culture without FBS. Conclusions The RA-induced PC12 cells expressing PS-1 mutant L286V shows that the proliferation is inhibited and blocked in the G1→S phase. Whether with or without FBS, PS-1 mutant L286V can accelerate the apoptosis of RA-induced PC12 cells. This action is enhanced markedly under the culture without FBS.
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WPRIM
Language:
Zh
Journal:
Chinese Journal of Geriatrics
Year:
2003
Type:
Article