Effect of homocysteine on the expression of protooncogene and neointimal hyperplasia in rat carotid arteries after balloon injury / 中华老年医学杂志

ABSTRACT

Objective To study the effect of homocysteine(Hcy) on the expression of c-fos and c-jun mRNA and neointimal hyperplasia in rat common carotid artery, and to explore the possible mechanism of homocysteine exacerbating neointima formation after balloon injury. Methods The mRNA expression of c-fos and c-jun were detected by semi-quantitative RT-PCR in the common carotid artery. The morphology of arterial intima and media was studied by optical microscopy and image analysing system. Results The mRNA expression of c-fos and c-jun was significantly elevated in the low methionine(LM)〔(1.40?0.21),(1.43?0.25)〕 and high methionine(HM) 〔(1.68?0.27),(1.71?0.30)〕 than that in the control group 〔(1.10?0.15),(1.00?0.13), P