Adenovirus-mediated transfer of MC148 gene to antagonize the chemotaxis of monocyte to MCP-1 / 中华器官移植杂志

ABSTRACT

Objective To study the antagonism to chemokine MCP-1 of MC148 gene which homogued human?chemokines mediated by adenoviral vector.Methods MC148 was amplified from DNA of molluscum contagiosum virus (MCV) isolates and sequenced. The product was co-tranfected into adenovirus vector to construct recombinant Ad-MC148. MC148P protein was obtained from the supernatant of 293 cells transfected with recombinant Ad-MC148 and served as antagonist against the recruitment of monocyte to MCP-1 in chemotaxis inhibition assay.Results MC148 gene was cloned, the sequence of which was identical to that reported in GenBank. Recombinant Ad-MC148 was constructed successfully. MC148P could antagonize the chemotaxis of monocyte to MCP-1. Conclusions MC148 could antagonize chemokine MCP-1 and inhibit chemotaxis of monocyte. MC148 is an antagonist against MCP-1. This study might provide a novel way to control immune response.