Expression of prostate stem cell antigen in human prostate cancer and its significance / 中华泌尿外科杂志

ABSTRACT

Objective To investigate the expression of prostate stem cell antigen(PSCA) in human prostate cancer (PCa) specimens and its possible correlations with pathological grade and clinical stage of PCa. Methods Immunohistochemical (IHC) and in situ hybridization (ISH) analyses of PSCA expression were simultaneously performed on paraffin-embedded sections from 20 cases of benign prostatic hyperplasia (BPH),20 cases of prostatic intraepithelial neoplasm (PIN) and 40 cases of (PCa) tissues.The levels of PSCA protein mRNA expression were semiquantitatively scored by assessing both the percentage and intensity of PSCA-positive staining cells in the specimens,and then were compared among PCa,BPH and PIN tissues. Results The moderate to strong positive rate of expression of PSCA protein and mRNA in PCa,BPH and PIN tissue was 85%,20% and 35%,respectively.Significant difference of expression was found between PCa and BPH,PIN samples (P0.05).The expression level of PSCA increased with higher Gleason score and worsening clinical stage (P