Construction and identification of mcpr1 gene eukaryotic expressing vector / 实用口腔医学杂志
Journal of Practical Stomatology
;
(6)2001.
Article
in Chinese
| WPRIM
| ID: wpr-540677
ABSTRACT
Objective:
To construct a high effective eukaryotic expre ss ing vector containing mcpr1 gene.Methods:
mcpr1 gene w as amplified by PCR from the plasmid T-easy/ mcpr1, then PCR product was in serted into eukaryotic expressing vector pcDNA3.1/V5-His B. The positive recomb inant was identified by PCR analysis, HindIII and BamHI restriction analysis and Sequence analysis.Results:
A 400 bp DNA fragment was amplified from the recombinant. Sequence analysis and restriction digest demonstrated tha t the mcpr1 gene was successfully inserted into pcDNA3.1/V5-His B plasmid.Conclusion:
The eukaryotic expressed vector pcDNA3.1/V5-His B/ mcpr1 has been successfully reconstructed.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Journal of Practical Stomatology
Year:
2001
Type:
Article
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