Genitourinary phenotype of androgen receptor knockout mice / 中华泌尿外科杂志

ABSTRACT

Objective To investigate the genitourinar y phenotype in androgen receptor knockout (ARKO) mice. Methods Using Cre-lox technique, the female Flox-AR mice were crossed with male ACTB-Cre mice,and the genotype of their filial generations was confirmed by PCR .By screening,5 ARKO mice were generated as study subjects and 5 wild type mice served as controls.The genitourinary phenotypes of the 2 groups were compared.Th e anal genital distance and testis weight were measured;serum testosterone and e stradiol levels were assayed by ELISA. Results The anal genital distance in ARKO group was (0.5?0.1)cm,while it was (1.1?0.1)cm in wil d type group.The prostate,seminal vesicle, epididymis and spherical cavernous bo dy muscle were absent in ARKO group with the reduced testis weight of (0.006 ? 0.001)g;while those were normal with the testis weight of (0.086?0.002)g in w ild type group.The serum testosterone level in ARKO group was (0.056?0.045)nmol /L,and it was (0.843?0.736)nmol/L in wild type group.The serum estradiol level in ARKO group was (1390.1?294.3)pmol/L,and it was (786.2?150.8)pmol/L in wild type group.The differences were statistically significant between the 2 groups ( P