Immunogenicity of Porphyromonas gingivalis gene vaccine pcNDA 3.1(+)/kgp_(cd) / 实用口腔医学杂志
Journal of Practical Stomatology
;
(6)1996.
Article
in Chinese
| WPRIM
| ID: wpr-540972
ABSTRACT
Objective:
To evaluate the immunogenicity of the recombinant plasmid pcDNA 3.1(+)/kgp_ cd.Methods:
BALB/c mice were immunized with recombinant plasmid pcDNA 3.1(+)/kgp_ cd,KGP_ cd protein(control) or vector pcDNA 3.1(+)(control) by quadriceps injection or targeted submandibular gland(TSG) injection. Serum IgG and salivary sIgA levels were assayed by indirect ELISA after immunization. The expression of the protein KGP_ cd in quadriceps and submandibular gland was detected by immunohistochemistry techniques.Results:
Serum specific anti-KGP_ cd IgG elicited by pcDNA 3.1(+)/kgp_ cd and KGP_ cd protein was significantly higher in both the quadriceps injection group and the TSG group than that in the pcDNA 3.1(+) group (P
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Practical Stomatology
Year:
1996
Type:
Article
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