Reversal of multidrug resistance by neferine in human gastric carcinoma cell line SGC7901/VCR / 中国癌症杂志

ABSTRACT

Purpose:To study the reversal of multidrug resistance (MDR) and its mechanism by neferine which is a new calcium channel blocker in human gastric carcinoma cell line SGC7901/VCR. Methods:The cytotixic effect was tested by MTT assay. The expression of multidrug-resistance-associated protein in human gastric carcinoma cells was examined by SP immunocytochemical and flow cytometry. Results:Neferine at the concentration 10 ?mol/L was not of significant cytotoxicity to SGC7901 and SGC7901/VCR cells. Neferine at the concentration of 2.5,5,10 ?mol/L decreased the IC_(50)value of VCR to SGC7901/VCR cells from 2.32 ?g/ml to 0.340,0.128 and 0.053 ?g/ml, respectively and with the increase by 6.8-,18.1-and 43.8-fold in the chemosensitivity, respectively. It had more potent reversal action on SGC7901/VCR cells than Verapamil at the concentration of 10 ?mol/L(P