The effect of calcitonin gene-related peptide on the expression of osteoprotege rin and receptor activator of nuclear factor-?B ligand mRNA in rabbit osteobla sts / 实用口腔医学杂志

ABSTRACT

Objective:To elucidate the effects of calcitonin gene-re la ted peptide(CGRP) on the gene expression of osteoprotegerin(OPG) and receptor ac tivator of nuclear factor-?B ligand(RANKL) in rabbit osteoblasts. Meth odsOsteoblasts were cultured in media containing 10 -10～10 -7 mol/L of CGRP. After 24-hour incubation,semi-quanitative RT-PCR was perfor med to detect the expression of OPG and RANKL mRNA,and with ?-actin mRNA as th e internal control. Results:CGRP increased the mRNA expressio n of OPG with the maximal effect at the concentration of 10 -8～10 -7 mol/L. CGPR downgulated the mRNA expression of RANKL dose-dependantly.C onclusionCGRP may regulate the activities of osteoclasts by regulating gene expression of OPG/RANKL.