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Research on PHA-CIK cells' immunophenotype and cytotoxicity / 中国免疫学杂志
Chinese Journal of Immunology ; (12)1985.
Article in Chinese | WPRIM | ID: wpr-541327
ABSTRACT

Objective:

To research PHA-CIK cells' phenotype and cytotoxicity.

Methods:

Using phytohemagglutinin(PHA)to stimulate peripheral blood mononuclear cells(PBMNCs) for 24 h,then cultured like incubating cytokine induced killers by traditional method. On 15th day examined its immunophenotype by flow cytometry and using MTT method to evaluate cytotoxicity.

Results:

The ratio of CD3+,CD8+, CD3+ CD8+ and CD3+ CD56+ cells were high.PHA-CK cells cytotoxicity was strong in some degree.

Conclusion:

PHA-CIK cells had strong cytotoxicity.The result provides an experimental basis for biotherapy.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Immunology Year: 1985 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Immunology Year: 1985 Type: Article