Preliminary study on human immune cells activated by CpG-ODN / 中国免疫学杂志
Chinese Journal of Immunology
;
(12)1985.
Article
in Chinese
| WPRIM
| ID: wpr-541332
ABSTRACT
Objective:
To study immunological stimulation of synthetic oligodeoxynucleotides(ODN) containing unmethylated CpG dinucleotides(CpG-ODN) on human immune cells.Methods:
CpG-ODN was co-cultured with peripheral blood mononuclear cells(PBMC) .The IFN-? and IFN-? in the supernatant were measured by ELISA;the reverse transcription PCR was used to analyze the expression levels of TLR9 mR NA in PBMC;MTT method was used to detect NK-mediated lytic activity of K562 cells.Results:
CpG-ODN induced high amounts of IFN-? as well as IFN-? production;the lysis of K562 mediated by CpG-ODN 2216 stimulating NK cells was increased;the CpG-ODN could up-regulate the expression of TLR9 mRNA in PBMC.Conclusion:
CpG-ODN 2216 can activate human immune reaction by increasing the production of IFN-? and IFN-?,the expression of TLR9 and NK cell lytic activity.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
1985
Type:
Article
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