Effects of genistein on proliferation of osteoblast of post-menopausal patients and the likely molecular mechanisms / 中华老年医学杂志

ABSTRACT

Objective This study was designed to explore the effects of phytoestrogen genistein on proliferation of post-menopausal osteoblast and the likely molecular mechanisms. Methods Osteoblast cultures were prepared from the upper femur of postmenopausal patients. Osteoblast proliferation was determined by Methyl Thiazolyl Tetrazolium(MTT)assay and cell cycle distribution by cytometry. The protein expressions of cyclin D and E were examined using Western-blot. Results Genistein (10~(-8) to 10~(-6) mol/L) stimulated cell viability of postmenopausal osteoblasts and increased the distribution ratio of the cells at the G2/M and S phases (P