Identification of 5-flank upstream regulation region of CD226 / 中国免疫学杂志
Chinese Journal of Immunology
;
(12)2000.
Article
in Chinese
| WPRIM
| ID: wpr-541750
ABSTRACT
Objective:
To identify the function of the 5′-flank upstream regulation region of human CD226 gene.Methods:
The upstream regulation region of CD226 was cloned by PCR and ligated into pGL3 vector. Then the vector was transfected into Jurkat cell and luciferase activity was detected after 48 h culture.Results:
CD226 gene may have two promoters, P1 and P2,which were located at the region of -843--319 bp and +1-+181 bp respectively, and PMA can up-regulate P1 while down-regulate P2. Both P1 and P2 can be up-regulated by A23187, especially P2.Conclusion:
CD226 gene may have two promoters, and PMA and A23187 can regulate CD226 promoter activity in the similar pattern of protein level.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
2000
Type:
Article
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