Construction and expression of IL-24 in eukaryotic expression vector and study of its antitumor effects / 中国免疫学杂志

ABSTRACT

Objective:To construct an eukaryotic expressing plasmid of IL-24,and investigate its inhibitory effects on the growth of tumor cells in vitro and in vivo.Methods:The eukaryotic expressing plasmid of IL-24(pEGFP-C1-IL-24) was constructed by DNA recombination technique.The recombination plasmid and empty vector were transfected into HIC cells,respectively with Lipofectamine 2000 Reagent and the expressions were determined by LSCM;the proliferation of HIC cells was measured by MTT assay;and apoptosis rate and cell-cycle distribution of HIC cells were measured with Flow Cytometry.Mice were inoculated with B16 cells,which were transfected with pEGFP-C1-IL-24 or empty vector,and the tumor size in mice was detected.The inhibitory effect of IL-24 gene transfection in mice solid tumor was observed and measured.Results:The expression of pEGFP-C1-IL-24 in HIC cells was determined by LSCM after the transfection of pEGFP-C1-IL-24.Comparing to the control group,the proliferation of HIC cells was inhibited by transfection with pEGFP-C1-IL-24 and the G2-M phase of the transfected cells was also increased.Moreover the percentage of mice with detectable tumor was significantly decreased after inoculated with B16 cells transfected with pEGFP-C1-IL-24.Growth rate of tumor in mice model was also significantly inhibited in IL-24 gene therapy group as compared with the control grouop(P