Inflammatory reaction and expression of adhesion molecules and cyclooxygenase-2 after reperfusion in focal cerebral ischemia rats / 中华神经科杂志

ABSTRACT

Objective To clarify the possible role of cyclooxygenase-2(COX-2), intercellular adhesion molecule-1(ICAM-1) and E-selectin in the inflammatory injury induced by cerebral ischemia and reperfusion. Methods The focal cerebral ischemia and reperfusion model was induced by a suture occlusion of the right middle cerebral artery. The rats were randomly assigned to four groups sham operated group; 4 h,22 h and 46 h reperfusion groups. The expression of ICAM-1, E-selectin and COX-2 were detected by immuno-Western blot and immunohistochemical method. Myeloperoxidase (MPO) activity was detected by a commercial MPO kit. Results In the right cortex and striatum of the sham operated group and the 4 h, 22 h and 46 h reperfusion groups, the relative values of COX-2 were 0.7642?0.0763, 1.5382?0.1047, 1.6491?0.3265, 1.8020?0.3719 and 0.7104?0.0891, 2.2061?0.2143, 1.7897?0.3537, 1.8018?0.5703 respectively; the relative values of ICAM-1 were 0.6845?0.0531, 0.9115?0.0422, 0.9426?0.0407, 1.0756?0.0467 and 0.6583?0.0361, 0.9439?0.0746, 0.9975?0.1532, 0.8808?0.0497 respectively. Significant increase of MPO activity and expression levels of COX-2, ICAM-1 and E-selectin were shown from 4 h to 46 h in the striatum and cortex of rats following reperfusion. Immunohistochemical staining showed that the number of ICAM-1 and E-selectin positive vessels was increased in the border of ischemia. The positive cells of COX-2 were almost neurons in the cortex, but in the striatum, the cells were neurons, glias and vessel endothelium. Positive correlation between COX-2 and ICAM-1 expression in the cortex(n=6,r=0.973,P