Clinicopathologic features and DNA ploidy of sporadic colorectal cancers with microsatellite instability / 中国癌症杂志

ABSTRACT

Purpose:To explore the clinicopathologic features of sporadic colorectal cancer patients with microsatellite instability(MSI) and the correlation between the microsatellite instability and the DNA ploidy.Methods:Detecting microsatellite instability with two sites(BAT25 and BAT26),conduct flow cytometry to analyze the DNA ploidy among 71 sporadic colorectal cancer patients and probe into the relationship between the microsatellite instability phenotype and the clinicopathologic characteristics as well as the DNA ploidy.Results:The positive rate of the microsatellite instability among sporadic colorectal cancer patients was 9.86 percent.The microsatellite instability phenotype was correlated with the tumor site and the histopathologic type and the differentiation grade with the P value less than 0.05,while it was independent of the gender,age,lymph node metastasis and Dukes' stage.The proportion of right colon cancers and poorly-differentiated adenocarcinomas of sporadic colorectal cancers showing microsatellite instability was higher than that of microsatellite stable ones.18 and 50 cases showed diploidy and aneuploidy respectively.5 cases of MSI were diploid,There was statistically significant correlation between the microsatellite instability phenotype and the DNA ploidy(P=0.012).Conclusions:Sporadic colorectal cancers with microsatellite instability were prone to occur in the right colon and to be poorly-differentiated adenocarcinomas and inclined to be diploid.