Study on the effects of the lymphocyte proliferation immunized with rHBsAg and CpG ODN in mice / 中国免疫学杂志

ABSTRACT

Objective:To investigate the effects of lymphocyte proliferation using the synthetic oligodeoxynucleotide containing CpG motif(CpG ODN) as immunal adjuvant immunized with the recombinant hepatitis B surface antigen(rHBsAg) in mice.Methods:BALB/c mice were randomly divided into five groups,injected into back leg tibialis anterior(TA) muscle for two times.The spleen and thymus lymphocytes proliferation was tested by MTT and()~3H-TdR incorporation respectively.Results:Analytical study showed that all groups compared with control the proliferation of spleen and thymus lymphocytes was increased obviously,except the specific proliferation of thymus lymphocyte there didn't obviously difference in CpG ODN plus vaccine group and the vaccine group.Meanwhile,the proliferation of the groups plus CpG ODN was much stronger than the not-CpG ODN groups in all the tests.Conclusion:CpG ODN has the ability stimulating the lymphocyte proliferation in mice obviously anti could have potential adjuvant effectiveness.