Reproducibility of Total T Lymphocyte and Characterization of CD3(+)/CD4(-)/CD8(-) Lymphocyte in T Lymphocyte Subset Assay / 대한임상병리학회지
Korean Journal of Clinical Pathology
;
: 119-124, 2002.
Article
in Korean
| WPRIM
| ID: wpr-54209
ABSTRACT
BACKGROUND:
Total T lymphocytes can be measured by CD3-fluorescein isothiocyanate (FITC)/CD4-phycoerythrin (PE) and CD3-FITC/CD8-PE. The difference in the CD3 percentages between these two determinations was evaluated. And, we characterized the CD3(+)CD4(-)CD8(-) T lymphocytes subset using the monoclonal antibody that detects gamma delta T lymphocytes receptors.METHODS:
The T lymphocyte subset assay was performed on 221 samples. A two-color direct immunofluorescence flow cytometric assay was done using a Simultest IMK-Lymphocyte kit (Bec-ton- Dickinson, San Jose, CA, USA). If the difference between the CD3 determinations were greater than 3%, the entire procedure was reviewed and the flow cytograms were reanalyzed. In 71 among 221 samples the proportion of gamma delta T lymphocytes was determined.RESULTS:
The difference between the CD3-FITC/CD4-PE tube and CD3-FITC/CD8-PE tube was 3.0%, 3.6%, 3.0%, 3.4%, and 2.4% in normal subjects, patients with chronic liver disease, patients with cancer, patients with other diseases, and children, respectively. The between-tube differences for CD3 exceeding 3% were found in 69 samples (31.2%). The proportion of gamma delta T lymphocytes was 0.81%, 2.46%, 2.50%, and 0.85% in normal controls, patients with chronic liver disease, patients with cancer and patients with other diseases, respectively. No correlation between gamma delta T lymphocytes and CD3(+)CD4(-)CD8(-) T lymphocytes was observed.CONCLUSIONS:
The reproducibility of the total T lymphocytes should be improved because of the between-tube difference exceeding 3% in about one third of the cases. Additionally, CD3(+)CD4(-)CD8(-) T lymphocytes were composed of heterogeneous subsets including gamma delta T lymphocytes and their proportion might be considered to be related to individual variation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Lymphocytes
/
T-Lymphocytes
/
Lymphocyte Subsets
/
Fluorescent Antibody Technique, Direct
/
Liver Diseases
Limits:
Child
/
Humans
Language:
Korean
Journal:
Korean Journal of Clinical Pathology
Year:
2002
Type:
Article
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