Relationship between Mutans streptococci transmission from mothers to children and initial adherence properties / 实用口腔医学杂志

ABSTRACT

Objective:To investigate the relationship between Mutans streptococci (MS) transmission from mothers to children and its initial adherence properties.Methods:200 MS isolates were genotypied by AP-PCR to demonstrate transmission between 20 pairs of mother and child aged 3~4 years, and to detect the transmitted strains and non-transmitted strains of mothers. Then the adherence of the strains to salivary coated hydroxyapatite beads (SHA) were determined by 3H- thymidine incorperation assay.A restriction fragment length polymorphism (RFLP) study of the different regions(spap-a,spap-pv)of the spap were undertaken by endonuclease haeⅢ and AluⅠrespectively. Results:The transmitted strains showed weaker adherence properties than the non-transmitted strains (P