Experimental study of Yanshu injection acted on SGC-7901,HepG2 and BEL-7402 tumor cell / 肿瘤研究与临床

ABSTRACT

Objective To discuss the clinical mechanism of Yanshu injection by observing its function to the tumor cells SGC-7901,HepG2 and BEL-7402 in vitro.Method The kill rate by MTT,flow cytometry were used to detect the change of cell cycle and apoptosis rate,and the expression of bcl-2,CD44V6 and nm23 of HepG2,SGC-7901 and BEL-7402 were observed.Results In vitro,to HepG2,SGC-7901 and BEL-7402,the living rate after using Yanshu injection was 72 %,40 % and 50 %.Compared with the control,the cell quantity in S phase decreased,and the apoptosis rate was higher,the expression of CD44V6 and nm23 changed a lot.Conclusion To the tumor cells,HepG2,SGC-7901 and BEL-7402,Yanshu injection can kill them,arrest them in G2 phase,block the conversion to M phase,accelerate cell apoptosis which has no correlation with the expression of bcl-2,and it still can restrain tumor metastasis.