THE GROWTH EFFECT OF TECTAL EXTRACT ON NEONATAL RAT RETINAL NEURONS CULTURED ON VARIOUS SUBSTRATA / 解剖学报

ABSTRACT

The retinal cells from 3-4 days neonatal rats were isolated and cultured on human amniotic basement membrane (HABM), laminin (LN), and polyornithine (PO) as the substrata. We have studied the morphology of these cultures and quantified the survival and growth of these cells in the absence and presence of tectal extract (Te) by MTT automatic colorimetric microassay. Neurons were identified by using the neuron specific enolase (NSE) immunocytochemical staining. The results showed that after 48 hrs culturing, some retinal cells grew larger and more actively on HABM, LN and PO in the presence of Te as compared to the controls without Te; the number of retinal cells with diameter above 10 ?m grown on HABM and LN with Te had increased 3 times compared to the controls without Te. MTT optical density (OD) results indicated that between the cell density range from 7?10~4 to 1.2?10~5 per well, the retinal cell cultures with Te gave significantly higher OD values than those controls without (P