Isolation,purification and identification of human periodontal ligament stem cells / 实用口腔医学杂志
Journal of Practical Stomatology
;
(6)2001.
Article
in Chinese
| WPRIM
| ID: wpr-542467
ABSTRACT
Objective:
To isolate and identify the periodontal ligament stem cells.Methods:
Periodontal ligament tissues were digested with a mixture of collagenase and Dispase,then the periodontal ligament stem cell clones were isolated by limited dilution method.The transmission electron microscopy(TEM),flow cytometery and immunohistochemistry procedure were employed to study the ultrastructure,cell cycle and surface marker of the cloned cells.Results:
The obtained cells showed the characteristics of undifferentiated morphology at ultrastructural level.94.4% of the cells were in phase G_0/G_1.The cells were Vimentin,STRO-1,ON and BSP positive.Conclusion:
Cloning incubation may be the effective way to isolate and purify the periodontal ligament stem cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
/
Prognostic study
Language:
Chinese
Journal:
Journal of Practical Stomatology
Year:
2001
Type:
Article
Similar
MEDLINE
...
LILACS
LIS