Effect of vitamin A deficiency on incidence of DMH-induced nephroblastoma in rats / 中华泌尿外科杂志

ABSTRACT

Objective To study the effect and the mechanism of vitamin A deficiency (VAD) on neoplasia of nephroblastoma induced by dimethylhydrazine dihydrochloride (DMH). Methods A total of 90 Wistar rats weighing 40-50 g were divided into 3 groupsnormal diet (ND) group (n=30),VAD group (n=30) and control group (n=30).In ND and VAD groups, 0.35% DMH was injected subcutaneously and the animals were fed with normal diet in ND group, and with vitamin A deficient diet in VAD group.In control group,the animals were fed with normal diet without DMH. The incidence of nephroblastomas and nephrogenic rests (NRs) in 3 groups were recorded. The renal tissues were prepared for detection of DNA index (DI) and S fraction of cell cycle by FCM,and for RAR? mRNA expression by RT-PCR.The incidence of nephroblastomas and NRs were determined according to Beckwith diagnostic criteria. Results No tumor developed in control group; the incidence of nephroblastoma in VAD group (36.7%) was significantly higher than that of ND group (13.3%,P