IP10 enhances anti-tumor immunity and its mechanisms / 中国免疫学杂志

ABSTRACT

Objective:To investigate the effects of IP10(IFN-? inducible protein 10,CXCL10) on anti-tumor immune response and to explore its mechanisms involved in.Methods:A mammary carcinoma cell line 4T1 was transfected with pcDNA3-IP10(IP10-4T1) by electrophoration and positive clones were screened in the presence of G418.Growth kinetics of IP10-4T1 cells was observed in vitro and in vivo.Survival rate among the animals was determined by daily assessment.Proliferation activity of lymphocytes was analyzed with()~3H-TdR incorporation.The phenotypes of lymphocytes isolated from tumors by ficoll density gradient centrifugation were assayed by flow cytometry.Results:The growth rate of IP10-4T1 cells was similar with that of parental 4T1 cells and neo-vector transfected 4T1 cells in vitro.Growth of the tumors formed by IP10-4T1 cells was inhibited in vivo.Compared to those of controls,the size and the weight of the tumors formed by IP10-4T1 cells decreased significantly 35 days post tumor transplantation(P