Study of the differentiation of NSCs induced by serum free condition medium of OECs from adult rat / 中国矫形外科杂志

ABSTRACT

[Objective]AIM To explore the differentiation of neural stem cells from rat embryonic spinalcord in the serum free condition culture medium of OECs.[Method]OECs were acquired by dissection ofolfactory nerve ensheathing and cultured for 20 hours,then suspending cells were put intoselective culture medium and cultured in the fibernectin coated plate.The activity of OECs was detected by MTT method on different periods and the best situation was selected out and serum-free cultured.Exudates of OECs was added into neural stem cells of the third generation.The differentiation of neural stem cells were observed under inverted microscope.The neural stemcells cultured were identified by immumofluorescence method.[Result]The activity of OECs was high on the 9~(th) day and the 12~(th) day.The serum free condition culture medium of OECs induced 53% neuralstem cells to differentiate into neuron-like cells and 42% neural stem cells into astrocytes cells.[Conclusion]The activity of OECs on different periods is different.The serum free condition culture medium of OECs can obviously induced neural stem cells to differentiate into mature neuronecells.