Cultivation of human bone marrow mesenchymal stem cells and induced differentiation into neuron-like cells / 中华器官移植杂志
Chinese Journal of Organ Transplantation
;
(12)1996.
Article
in Chinese
| WPRIM
| ID: wpr-542566
ABSTRACT
Objective To explore a way that induce human bone marrow mesenchymal stem cells to differentiate into neuron-like cells in vitro.Methods Bone marrow mesenchymal stem cells were cultivated in a medium containing serum by using density gradient centrifugation method and whole marrow method and were induced to differentiation by ?mercaptoethanol.Immunohistochemistry were used to identify the type of cells.Results The number of cells was((1.73)?(0.44))?10~(7)/ml by using whole marrow method and ((7.65)?(0.52))?10~(7)/ml by using density gradient centrifugation method at the 10th day cultivation respectively.There was statistically significant difference(P
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Organ Transplantation
Year:
1996
Type:
Article
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