A Study on the Genetic Polymorphisms of Glutathione-S-transferase P1 and M1 in Hakka Population of Meizhou Area in Guangdong, China / 环境与健康杂志

ABSTRACT

Objective To investigate the distribution of genetic polymorphisms of human glutathione-S-transferase P1 and M1(GSTP1 and GSTM1) in Hakka population of Meizhou area in South China. Methods Co-amplifying GSTM1 and ?-Globin gene to detect the absence or presence of the GSTM1 gene, polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) were used to identify the genotypes of GSTP1 gene,and the data were analyzed with SPSS10.0 software. Results The GSTP1 and GSTM1 genetic polymorphisms were examined in 512 samples. The frequency of null genotype of GSTM1 gene was 62.1%, while the frequency of GSTP1 A/A, GSTP1 A/G and GSTP1 G/G were 69.1%, 28.2% and 2.7% respectively, and the GSTP1 genetic polymorphism distribution was in accordance with the Hardy-Weinberg equilibrium rule. A significant difference was observed in GSTM1 genetic polymorphisms between drinkers and non-drinkers. The drinkers had higher frequency of absence GSTM1 gene. There was also a significant difference in the GSTP1 genetic polymorphisms between smoking group and non-smoking group. Compared with non-smokers, the smokers had a higher frequency of mutate genotype of GSTP1 gene and lower frequency of wild genotype. However there were no differences shown on the frequency of the GSTP1 genotypes in the aspect of cumulative tobacco consumption. Logistic regression analysis results showed that there was a weak association between the GSTM1 genetic polymorphisms and family history of hypertension. Conclusion There are significant differences in the GSTP1 genetic polymorphisms among different smoking status and in GSTM1 genetic polymorphisms between drinkers and non-drinkers. A weak association has been observed between the GSTM1 genetic polymorphisms and family history of hypertension.