Effects of interleukin-18 on transdifferentiation in renal tubular epithelial cells / 中国免疫学杂志
Chinese Journal of Immunology
;
(12)1986.
Article
in Chinese
| WPRIM
| ID: wpr-542920
ABSTRACT
Objective:
To study the effect of interleukin-18 on transdifferentiation in renal tubular epithelial cells(TECs).Methods:
Human proximal tubular epithelial cell line(HK-2) was cultured in vitro.TECs were exposed to different concentrations(0,0.1,1,10 and 100 ng/ml) of IL-18 for 24,48 and 72 hours.At the end of each incubation,the expressions of the ?-SMA and TGF-?_1 mRNA were assessed by RT-PCR,the rate of ?-SMA expressing TECs was assessed by immunocytochemistry,and the expression of the ?-SMA protein was assessed by Western blotting.Results:
(1)The expressions of ?-SMA and TGF-?_1 mRNA were increased significantly by a dose- and time-dependent manner when TECs were exposed to IL-18(P
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
1986
Type:
Article
Similar
MEDLINE
...
LILACS
LIS