Expression and signififcance of IGF-Ⅰ TGF-?1 in human mesenchymal stem cells after exposured to extracoporeal shock wave / 中国矫形外科杂志
Orthopedic Journal of China
; (24)2006.
Article
in Zh
| WPRIM
| ID: wpr-543432
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WPRO
ABSTRACT
[Objective]To demonstrate mechanism of ESW in curing osteogetic disorders,we studied expression of some osteogenetic factors in human mesenchymal stem cells(hMSCs)when exposed to ESW.[Method]After success in marrow aspiration,isolation and obtainment optimal experimental energy,a dose of 5kV and 100 times of ESW was applied to hMSCs of passage 1.The expression of IGF-Ⅰ and TGF-?1 were examined by immunocytochemical staining.[Result]The cytochemical staining results showed that expression of IGF-Ⅰ and TGF-?1 appeared at different passage of hMSCs after ESW intervention.Appearance of IGF-Ⅰ was earlier than TGF-?1 which didnt express until passage 7.At the same interval,the expression of IGF-Ⅰ and TGF-?1 in control group difference is lower than ESW group,respectively(P
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Language:
Zh
Journal:
Orthopedic Journal of China
Year:
2006
Type:
Article