Analysis and improvement of methylation-specific polymerase chain reaction / 肿瘤研究与临床
Cancer Research and Clinic
;
(6)2006.
Article
in Chinese
| WPRIM
| ID: wpr-543920
ABSTRACT
Objective To analyze and improve Methylation-specific Polymerase Chain Reaction. Methods Methylation state of DAPK in gastric cancer was detected by this improved MSP. Results 100 % of frozen tissues and 24.1% of paraffin-embedded tissues could get either methylated production or unmethylated production. Conclusion Improved MSP is a convenient, sensitive and specific way to screen DNA methylation, especially for frozen tissues, but for paraffin-embedded tissues,it may be unsuitable.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Cancer Research and Clinic
Year:
2006
Type:
Article
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