Th1 cellular immune response elicited by recombinant adenovirus transduced dendritic cells for HCV-NS3 in vivo / 中国免疫学杂志
Chinese Journal of Immunology
;
(12)2001.
Article
in Chinese
| WPRIM
| ID: wpr-543975
ABSTRACT
Objective:
The aim of this study was to explore the vaccine potential of adenovirus transduced DC encoding for HCV nonstructural protein NS3 in a murine model.Methods:
Dendritic cells were isolated from bone marrow of BALB/c mice and cultivated with cytokine cocktail for 7 days. Recombinant adenovirus was added to DC cultures at day 7,at a titration of 2?10~9 pfu/10~6 DC/well. FACS and Western blot techniques were used to identify the DC mature state and the protein expression, respectively. BALB/c mice were primed/boosted immunized with DC based vaccines at 3?10~5 cells for one mouse at a 10 day interval. 10 days after the second immunization, cytokines secretion and IFN-? generating T cells response were evaluated by ELISA and ELLISPOT analysis.Results:
Our current data indicate that AdNS3 can stimulate the maturation of DC and efficiently express authentic HCV nonstructural proteins in infected cells. BALB/c mice inoculated with AdNS3 transduced DC twice generated strong IFN-? secreting T cells response(P
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
2001
Type:
Article
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