Effect of Aire on the production of CD4~+CD25~+ regulatory T cells / 中国免疫学杂志

ABSTRACT

Objective:To clarify the role of Aire on the production of regulatory T cells on mice.Methods:The distribution of CD4~+CD25~+T cells and the expression of Foxp3 mRNA from Aire~ -/- thymus and spleen were separately analyzed using FACS and real-time PCR.Results:Compared with control mice, the total numbers of thymocytes/splenocytes and T cells from Aire~ -/- mice did not show any significant changes; The total CD4~+CD25~+ regulatory T-cell number and Foxp3 mRNA expression did not exhibit any statistically difference; The ratios of CD4~+CD25~+T cells out of CD4~+T cells were similar based on adult /day3/day7 mice analysis.Conclusion:Aire gene does not affect the production of CD4~+CD25~+ regulatory T cells.