Study on the animal model of vIL-10 transgenic therapy for rabbit arthritis / 中国免疫学杂志

ABSTRACT

Objective:To establish a local ex-vivo gene transfer method to treat RA through animal experiments in vivo and in vitro using retrovirus(rV) as a vector which carrying rRV-vIL-10 target gene.Methods:①The rabbit RA models were induced by the rabbit synovial fibroblast cell line which could continuously expreesed hIL-1?. ②In vivo, the rabbit synovial fibroblast cell line was transduced with rRV-vIL-10, then adding G418 to pick out the rRV-vIL-10 positive clon and infecting the rabbit synovium through intra-articular injection. ③RT-PCR, IHC methods were performed to prove the success of gene transfer to the rabbit synovium and expressed the target protein. ④The relative cytokins changes were detected by ELISA before and after gene therapy and evaluated treatment efficacy of rRV-vIL-10.Results:①rRV-vIL-10 was a effective vector which could transfect to the rabbit synovium in vivo through RT-PCR and IHC methods. ②Intra-articular local gene therapy could effectly reduce the synovium inflammation level of rabbit joints and expressed mRNA and vIL-10 protein. The level of cytokin such as IL-1? was decline.Conclusion:Retrovirus-mediated transgene of vIL-10 is successfully transfected to the rabbit synovium ex-vivo and can reduce arthritis inflammation levels of the IL-1? induced arthritis.